Konnis

FAQ

Frequently Asked Questions About Automation

 

What do the numbers after the Tecan model stand for?

The names of Tecan instruments are typically followed with 75, 100, 150, or 200.  Example: Freedom EVO 150

The number represents the deck width in centimeters.  For the above example the instrument is 150 cm in width.

 

Why buy a 150 over a 100?

The compact size of the 100 is initially appealing especially when lab space is at a premium.  But once the scientist starts to use the instrument it frequently gets too small of what they find they can do with it.  The configurability of robotic instruments lend themselves for use by more than one process.  Different processes can require different automation devices as well as specific carriers.  In addition, the price differential when purchasing from Konnis is a small fraction to go from a 100 to a 150, so the decision is typically obvious.  Therefore unless there is only one specific use the instrument will ever be used for, Konnis recommends going with the 150 as a good compromise between functionality and space.

 

What does RSP mean in the Genesis model name?

Robotic Sample Processors

 

What does SBS format mean? and why do I care?

SBS Footprint, Stands for “Society for Biomolecular Screening”. Dimensions required of a microplate that is considered to meet the standards. Using standard sized carriers and adapters help to ensure interchangeability.

ANSI/SLAS 1-2004: Microplates — Footprint Dimensions

Length 127.76 mm ± 0.25 mm (5.0299 inches ± 0.0098 inches)

Width 85.48 mm ± 0.25 mm     (3.3654 inches ± 0.0098 inches)

Konnis manufactures highly precise carriers and hardware for SBS footprint. We design our SBS footprint platforms to fit the largest tolerance range of microplates based upon the ANSI/SLAS 1-2004: Microplate – Footprint Dimensions.

384 well microplates need a more precise fit than 0.0196 inches (0.5mm), which is the tolerance range of ANSI/SLAS 1-2004: Microplate – Footprint Dimensions. The range of tolerance for SBS standard is quite large in the manufacturing world, if you would like a tighter fit of your labware, send us a microplate sample for an exact measured fit within 0.0001 inches.

 

What are the differences between an EVO1 and EVO2 ?  Do I care?

The EVO1 deck has a wider rail bed found on a Genesis RSP, Genesis, and EVO1 deck.  The EVO2 is a narrower rail bed.  This is important to know if your assay requires optional deck equipment that uses this rail.

 

When do I need to run EVOware Vs. Gemini ? What are the differences?

Gemini has been observed to be more responsive than EvoWare and is less expensive.  It runs on both the Genesis as well as the EVO platforms as long as you are aware of the next point.

  • The MCA96 and PosID3 both require EvoWare.

A benefit to EvoWare is that it is more modular which can be helpful when adding devices and additional assays to the instrument.

 

Can I upgrade my instrument software from Gemini to EVOware?

Yes.  All your assays should transfer over to the newer application.  Konnis can assist in this process to assure a smooth transition.

 

What is the difference between a liquid instrument and an air instrument?

The main difference is that one uses liquid (typically DI water) as displacement to create a vacuum to pipette where as the air instrument uses air.

Performance:  The air system can pipette lower volumes with more accuracy, typically under 5ul.  Although liquid systems can perform well at 1 – 5ul depending on what is being pipetted and the accuracy required.

Pricing: The Air Liha is the newest model and is more expensive.

Maintenance:  There are fewer parts to replace when performing a PM on an air vs. a liquid instrument. It is worth discussing what the OEM recommends for the maintenance of the Air Liha arm.  Current information indicates replacement of the Air LiHa arm every other year.  It is best to obtain a quotation for the instrument as well as the following two years of maintenance to get the full picture of the system cost.

Why buy one over the other?

If your primary requirement is precision at a low volume i.e.  high level of accuracy at under 5ul volumes, the air will perform better.

 

Does my MCA 96 arm need to be replaced every 3 years?

Our 30+ accumulative years of  experience servicing Tecan instruments has shown that there is no general rule for the replacement of the MCA arm.  Many instruments have been in service for 10s of years with out arm replacement.  Consistent, quality maintenance is key to keeping the MCA as well as the entire instrument is good working condition preventing costly replacements of major components.  If the arm is not broken, or can simply be repaired there is no need to replace it.

 

How many deck pin locations are on the Tecan 100, 150, and 200?

30, 45, and 69 respectively

 

Can 1000 ul tips pipett lower volumes?

1000 ul tips can be used down to single ul pipetting depending on the characteristics of the fluid, but are recommended for 5 ul and above.  In general lower volume pipetting should utilize the smaller tips and low volume tubing.  If you have questions on what tip size to use please call us to discuss your specific scenario.

 

What are the dimensions of the extruded aluminum table that Konnis sells?

Our table is a few inches less in width than the instrument to allow for the instruments overhang.

Example the Konnis table for an EVO 150:    31”H x52W”x30” D

 

How does Konnis approach automating your assay?

  1. Obtain assay description
  2. Analyze assay by creating a flow chart of steps with timing
  3. Lay out demo of process with application software
  4. Develop automation proposal of process including required hardware
  5. Formulate quotation taking into account remote and on-site development time, carriers required, and consumables.
  6. Upon receipt of PO, develop script remotely
  7. Delivery of script, with on-site fine tuning

 

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