FAQ

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FAQ

Frequently Asked Questions About Automation

What does SBS (or SLAS/ANSI ) format mean? and why is it important?

SBS Footprint, Stands for “Society for Biomolecular Screening”. This form factor is also referred to as SLAS/ANSI form factor. Dimensions required of a microplate that is considered to meet the standards. Using standard sized carriers and adapters help to ensure interchangeability.

ANSI/SLAS 1-2004: Microplates — Footprint Dimensions

Length 127.76 mm ± 0.25 mm (5.0299 inches ± 0.0098 inches)

Width 85.48 mm ± 0.25 mm     (3.3654 inches ± 0.0098 inches)

Konnis manufactures highly precise carriers and hardware for liquid handling instruments. We design our SBS footprint platforms to fit the largest tolerance range of microplates based upon the ANSI/SLAS 1-2004: Microplate – Footprint Dimensions.

384 well microplates need a more precise fit than 0.0196 inches (0.5mm), which is the tolerance range of ANSI/SLAS 1-2004: Microplate – Footprint Dimensions. The range of tolerance for SBS standard is quite large in the manufacturing world, if you would like a tighter fit of your labware, send us a microplate sample for an exact measured fit within 0.0001 inches.

What are the steps to import or define new carriers on the Deck?

Evoware

  1. Exit EVOware™ software
  2. Copy the import file onto the PC desktop
  3. Double click on the import file to launch the EVOware™ import/export utility.
  4. Click on the carrier appearing in the right hand panel to select and highlight it.
  5. Click “Import” button to load the file.
  6. Once imported, click “OK” to confirm that a backup has been created.
  7. Click “OK” to confirm the import process.
  8. Exit the Import/Export utility and launch EVOware™. The new carrier will now be included in the list of available carriers.
  9. Drag onto the worktable and start using the new carrier.

Gemini

Can Konnis manufacture parts and consumables for my specific assay?

Yes, we offer full CNC machining capability, design and modeling using Solidwork®, as well as injection molding, 3D, printing, rapid prototyping,  laser marking and more.  If you are an OEM looking for a contract manufacturer to be able to manufacture and QC your parts contact us.

Do you offer nested tips for Liquid Handlers?

Yes, we have a few options for nested tips that can be used on your Liquid handler depending on the model.  Not all instruments can fit, or make full use of nested tips.  Our nested tip comes in a 200uL volume with a stack of 10 trays of 96 tips in the footprint of one deepwell plate.  The stack of tips can be placed on the deck of the system and the new and used trays of tips can be moved by use of a gripper arm on your instrument.  This makes very good use of space on the deck and can allow some instruments to fit more than 10,000 tips on the instrument.

When do I need to run EVOware™ Vs. Gemini™ ? What are the differences?

It runs on both the Genesis as well as the EVO platforms depending on the hardware options you may have installed on your system.  Its best to have a call to review.

  • The MCA96 and PosID3 both require EvoWare.

What are the dimensions of the extruded aluminum table for the Tecan® robots?

How many deck pin locations are on the Tecan® 100, 150, and 200?

30, 45, and 69 grids respectively

The numeric value denotes the width of the deck in centimeters – Example: Tecan® Freedom EVO 150 is 150 cm wide.

What is the difference between a liquid instrument and an air instrument?

The main difference is that one uses liquid (typically DI water) as displacement plunger along with an air gap to pipette where as the air instrument uses only air.  The fluid filled system allows you to pipette volumes based on the size of the syringe, as well as the ability to wash out the fixed tip canulas with a flow through process.

Can 1000 ul tips pipette lower volumes?

1000 ul tips can be used down to single ul pipetting depending on the characteristics of the fluid and what your acceptable %CV is, but are recommended for 10 ul and above.  In general lower volume pipetting should utilize the smaller tips and low volume tubing.  If you have questions on what tip size to use please call us to discuss your specific requirements.

Why buy one over the other?

The main difference is that one uses liquid (typically DI water) as displacement plunger along with an air gap to pipette where as the air instrument uses only air.  The fluid filled system allows you to pipette volumes based on the size of the syringe, as well as the ability to wash out the fixed tip canulas with a flow through process.

Does Konnis offer pipette tips compatible with Tecan®, Beckman®, Perkin Elmer® , and Agilent Bravo® instruments?

Yes.  We have pipette tips, microplates, troughs, and adapters that fit these instruments from the above manufacturers.  Typically, they are sold in case formats with the quantities differing based on the product in the case.  Volume order and standing order discounts are available.

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